… The lysates were then diluted in 1 ml of cold polysome buffer (25 mM Tris-HCl pH 7.5, 50 …
prepared in polysome buffer. Ribosomal pellets were rinsed briefly with polysome buffer, …

… Mayaro virus, O’nyong-nyong virus, Ross River virus, and Venezuelan equine encephalitis
virus. … The translation efficiency (ratio of viral RNA levels in the polysome fractions to those in …

… (S258D/S259D) while the phosphoinhibitory mutant (S258N/S259N) bound c-Jun mRNA
more. Examination of c-Jun mRNA distribution in polysome gradients in mutant cells further …

… During infection by (+)ssRNA virus, genomic RNA and/or its dsRNA replication products
are sensed by PRRs such as RIG-I, MDA5, TLR3, TLR7, and TLR8. RIG-I and MDA5 are …

… Emerging alphaviruses, such as chikungunya virus and Mayaro virus, … viral genomic RNA
(gRNA) and nucleocapsid (NC) formation. Instead, this results in decreased budding and virus …

… are more susceptible to heat stress. A very close mutualistic … global protein translation and
polysome disassembly. eIF2α … of viral replication complexes, but instead, G3BP is distributed …

… genome, over host RNA. The nature of nsP3 binding to viral RNA also appeared stochastic.
… applied to assess how nsP3 is able to recognise target RNA for binding. However, neither a …

… to the expansion of arbovirus distributions and the incidence of viral epidemics in novel …
stress granule native proteins to viral polysomes to remove an existing translational block while …

Positive single stranded RNA ((+) ssRNA) viruses pose a major health threat due to their
high mutation rates and adaptability. To counteract arising resistances against existing …

… through its CГterminal domain (CTD) to facilitate ;udding. CP is thought to interact with the
viral genome through … , although other polyanions can su;stitute for the viral genome 37 ?3=;8. …